Biological Nitrile Reduction ‐ Structure and Mechanism (LB136)
نویسندگان
چکیده
منابع مشابه
Unraveling the catalytic mechanism of nitrile hydratases.
To elucidate a detailed catalytic mechanism for nitrile hydratases (NHases), the pH and temperature dependence of the kinetic constants k(cat) and K(m) for the cobalt-type NHase from Pseudonocardia thermophila JCM 3095 (PtNHase) were examined. PtNHase was found to exhibit a bell-shaped curve for plots of relative activity versus pH at pH 3.2-11 and was found to display maximal activity between ...
متن کاملA dual site catalyst for mild, selective nitrile reduction.
We report a novel ruthenium bis(pyrazolyl)borate scaffold that enables cooperative reduction reactivity in which boron and ruthenium centers work in concert to effect selective nitrile reduction. The pre-catalyst compound [κ(3)-(1-pz)2HB(N = CHCH3)]Ru(cymene)(+) TfO(-) (pz = pyrazolyl) was synthesized using readily-available materials through a straightforward route, thus making it an appealing...
متن کاملSpectroscopic and Computational Studies of Nitrile Hydratase: Insights into Geometric and Electronic Structure and the Mechanism of Amide Synthesis.
Nitrile hydratases (NHases) are mononuclear nonheme enzymes that catalyze the hydration of nitriles to amides. NHase is unusual in that it utilizes a low-spin (LS) FeIII center and a unique ligand set comprised of two deprotonated backbone amides, cysteine-based sulfenic acid (RSO(H)) and sulfinic acid (RSO2-), and an unmodified cysteine trans to an exogenous ligand site. Electron paramagnetic ...
متن کاملMechanism and Biological Explanation
This article argues that the basic account of mechanism and mechanistic explanation, involving sequential execution of qualitatively characterized operations, is itself insufficient to explain biological phenomena such as the capacity of living organisms to maintain themselves as systems distinct from their environment. This capacity depends on cyclic organization, including positive and negati...
متن کاملDescartes ’ Mechanism and Biological Functions
The “new mechanical philosophy” takes its inspiration from biology and simultaneously traces its origin to René Descartes. It pays little attention to Descartes’ mechanistic biology, however, which, admittedly, even most historians of philosophy and science mistakenly treat as a straightforward extension of Descartes’ attempt to explain natural phenomena in terms of shapes and movement of parts...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2014
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.28.1_supplement.lb136